4 calculators in this category
- DNA / RNA Concentration from A260 CalculatorBeer–Lambert law with standard A260 conversion factors (Sambrook, Molecular Cloning)This calculator turns a 260 nm absorbance reading into the three numbers you actually need at the bench: concentration in…
- Primer Melting Temperature (Tm) CalculatorSantaLucia unified nearest-neighbour thermodynamics (1998)Paste a primer sequence and this calculator returns its melting temperature four ways: the nearest-neighbour thermodynamic…
- PCR Master Mix CalculatorC₁V₁ = C₂V₂ dilution arithmeticGive this calculator your reaction volume, how many reactions you are setting up, and the stock and final concentration of each…
- Delta Delta Ct Fold Change CalculatorLivak & Schmittgen 2^-ΔΔCt method; MIQE guidelinesEnter four Ct values — your target gene and your reference gene, each in the treated and the control sample — and this…
Which one do you need?
- DNA / RNA Concentration from A260 Calculator
- Convert an A260 absorbance reading into ng/µL, total yield in µg and molar concentration for dsDNA, ssDNA, RNA or oligos, with dilution and pathlength handled.
- Primer Melting Temperature (Tm) Calculator
- Paste a primer sequence to get its melting temperature by nearest-neighbour thermodynamics, the Wallace rule, GC and salt-adjusted formulas, plus annealing temperature.
- PCR Master Mix Calculator
- Build a PCR or qPCR master mix: per-reaction and total volumes for buffer, dNTPs, primers, MgCl2, polymerase and water, with pipetting excess included.
- Delta Delta Ct Fold Change Calculator
- Turn four qPCR Ct values into a fold change by the Livak 2^-ΔΔCt method, with an efficiency-corrected Pfaffl option, log2 fold change and an error sensitivity table.
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